TMSS Medical College Journal

Background: Eighty coagulase-negative Staphylococci (CONS) isolates from surgical wound, pus from infected skin lesions, burn exudates and diabetic ulcer exudates of patients in Mymensingh Medical College Hospital, Bangladesh, was evaluated in order to see their species distribution, pattern of antimicrobial resistance and virulence factors. Methods: The study was carried out in the department of Microbiology, Mymensingh Medical College during the period from July 2010 to May 2011 and it was a cross sectional descriptive type study. The cighty CoNS isolates were subjected to identification up to species level, antimicrobial resistance pattern and detection of virulence factors. Results: Out of 80 CoN isolates, 16 species were identified and S. epidermidis were the highest in number among them. Most of the isolates were resistant to oxacillin 36 (45%) and all were sensitive to imipenem and vancomycin. Virulence factor mecA gene was detected in 45 (56%) strains of CONS by PCR. No PVL gene was found. Conclusion: Considering various findings of this study, it can be concluded that CoNS are the emerging nosocomial pathogens in MMC. As MRCONS are multidrug resistant, therefore antibiotic sensitivity must be done prior to treatment in infections caused by them.

Staphylococci are gram positive, non motile bacteria that characteristically divide in more than one plane to form irregular cluster. Nearly all are facultative anacrobes that grow better under aerobic than anaerobic condition’. Staphylococci are widely spread in various niches such as clinical environments and food plants. Thirty-six validated described species, including 21 subspecies, belong to the Staphylococcus genus according to the List of Bacterial Names with Standing in Nomenclature, updated 3 December 20042. Species are classified as coagulase-positive Staphylococcus aureus and coagulase-negative staphylococci eg, Staphylococcus epidermidis, Staphylococcus saprophyticus.