SIMPLE NON-CULTURE METHODS FOR RAPID LABORATORY DIAGNOSIS OF URINARY TRACT INFECTIONS IN A TERTIARY CARE HOSPITAL
Abstract
Background: Urinary tract infections (UTIs) are among the most common bacterial infections and account for a
significant part of the workload in clinical microbiology laboratories. Physicians distinguish UTis from other discases
that have similar chnical presentations with diagnostic tests confusion. Objective: This study was undertaken to lind
out the simple and rapid diagnostic tests for Unnary tract mlections in tertiary care bospelal. Methodology: This cross
out the simple and rapid diagnostic tests for Unnary tract infections in tertiary care hospital. Methodology: This eross
sectional study was carried out in the Department of Microbiology at Enime Medscal College Trom December 2012 to
January 2014 for a period of 12 months. Specimens were process for microscopy and bacteria were isolated and
Identified according to standard laboratory procedure. Kesult: A total of 424 unne specimens were collected, Among
them 212 urine were showed culture positive (42.956), pyuria 272(55.0/7), bacteriuria 240 (48,6%) and both pyuria and
bacteruna 225(45.5%). Conclusion: It was concluded that the simple casy aid to diagnosis of Unnary tract infections
were microscopy for pyuria and bactenuna in comparison with time consuming bactenological culture.
Keywords
UTI. Pyuria
Bacteruria
Introduction
Urinary tract infections (UTls) are among the most common bacterial infections. It has been estimated that symptomatic U'Tis result in as many as 7 million visits to outpattent chines, million visits to emergency departments, and 100,000 hospitalizations annually!. UTIs have the MOst common hospital-acquired infection, accounting for as many as 35% of nosocomial infections, and they are the second most common cause of bacteremia in hospitalized patients. Physicians must distinguish UTI from other discases that have a similar clinical presentation, some Ulis are asymptomatic or present with atypical signs and symptoms, and the diagnosis of UTis in neutropenie patients (who do not typically have pyuria) may require different diagnostic criteria than those used for the general patient population" Several rapid screening tests are used commonly to make a presumptive diagnosis of UTI, among them nucroscopie examination of urine for pyura and bacteria. Gram stain of uncentrifuged urine specimens is a simple method Numerous studies have been published concerning the usefulness of these tests in diagnosing UTI$6, In fact, in many clinical laboratones, urine cultures are the mos? common type of culture, accounting for 24%-40% of submitted cultures; as many as 80% of these urine cultures are submitted from the outpatient setting?. Urine cultures are nocessary for outpatients who have rocurrent UTis, experience treatment failures, or have complicated UTIs Urine cultures are also necessary for inpatients developing Uris The hactenal culture remains an important test in the diagnosis of UTI, not only because it helps to document infection, but also because it is necessary for determination the identity the infecting microorganisms) and for antimicrobial susceptibility testing. The most commonly used enterion for delimina signiticant bacteruma is the presence of 105 ciu per milliliter of urines So, the aim of the study was designed to determine the rapid and easy laboratory procedure for diagnosis of UTI in tertiary care hospitals. arethral oritice before mictuntion, allowing the tirst part of the urine stream to pass into the toilet, and collecting urine for culture trom the mistream"